赵保路——中国科学院生物物理研究所研究员

赵保路,男，中国科学院生物物理研究所研究员，博士生导师，中国科学院研究生院教授。1970年毕业于中国科学技术大学，1981年研究生毕业于中国科学院研究生院，获硕士学位。曾先后在中国科学院生物物理研究所，美国 Ohio State University（1985-1987，1990-1992）和 University of California at Berkeley（1998-1999），英国 Institute of Food Research和香港大学（1996-1997从事研究工作。曾任中国科学院生物物理研究所副所长，主管业务工作。目前担任亚洲自由基研究学会主席，中国生物物理学会自由基生物学和医学专业委员会主任，中国衰老和抗衰老生物学委员会副主任，中国物理学会波谱学专业委员会理事，世界中医药学会联合会亚健康专业委员会理事，北京烟草学会常务理事。现为 “Biofactor”,“Journal of Clinical Biochemistry and Nutrition” “生物物理学报”,“波谱学杂志”,“中国老年学杂志”， 和“中国烟草学报”编委。先后完成“细胞膜膜蛋白的生物物理特性研究”，“癌症中活性氧的分子机理研究”，”香烟中瞬时和稳定有害自由基性质的研究" “巨嗜细胞产生一氧化氮自由基机理和天然抗氧化剂对一氧化氮的调节作用“和“氧自由基诱导神经细胞凋亡和天然抗氧化剂调节作用的分子机理“ 国家自然基金课题及 "氧自由基的代谢失衡和天然抗氧化剂的清除机理"和"氧自由基对细胞膜的损伤效应和天然抗氧化剂的调节机理”“活性自由基生物功能及其机理的ESR成像研究”和“植物抗病感病中一氧化氮自由基的作用机理”、“抗氧化剂预防帕金森症（PD）的机理研究”、“天然抗氧化剂延缓衰老作用机理”、和“烟碱预防和治疗老年退行性疾病研究”两项科学院和国家自然国家自然科学基金 85重点课题及多项国家基金课题。“肿瘤细胞膜的生物物理特性” 获 1987年中国科学院科技进步三等奖；“人多形核白细胞呼吸爆发产生的氧自由基和天然抗氧化剂的清除机理” 获1993年中国科学院自然科学二等奖。1998年“低毒低自由基香烟配方和生产方法”获中国科学院发明三等奖。先后在国内外核心期刊上发表200多篇研究论文，两部专著和 五项发明专利。现为国家基金“抗氧化剂通过PPARS延缓衰老的机理研究”，“光动力作用诱导癌细胞凋亡信号转导调控网络”和十五重大子课题“用核技术研究汞和稀土的分子毒理机制”项目和开发等项目负责人。和英国帝国理工学院有合作项目，与美国FDA生物物理实验室，The Salk Institute for biological Studies 神经实验室有合作项目。与法国Universite Victor Segalen-Bordeaux 2，神经生理实验室合作研究PD的发病机理和预防措施。同时与北京玄武医院老年病研究所有合作关系。2005年被美国“The Oxygen Club of California”授予终身会员称号。

近期发表的主要研究论文：

1. Shen J-G, Guo X-S, Jiang B, Li M, Xin W-J, Zhao B-L: Chinonin, a novel drug against cardiomyocyte apoptosis induced by hypoxia and reoxygenation. Biochim Biophyscs Acta 1500, 217-226,2000.

2. Shen J-Gang, Li M, Xin W-J, Zhao B-L: Effects of Chinonin on nitric oxide free radical, myocardial damage and arrhythmia in ischemia-reperfusion injury in vivo. Appl Magn Reson 19-9-19,2000.

3. Xin W-J, Wei T-T, Chen C, Ni Y-C, Zhao B-L: Mechanisms of apoptosis in rat cerebellar granule cells induced by hydroxyl radicals and effects of Egb761 and its constitutes. Txicology 148 103-110,2000.

4. Li, H-T, Hu J-G, Xin W-J, Zhao B-L: Production and interaction of oxygen and nitric oxide free radicals in PMA stimulated macrophages during the respiratory. Redox Report 5，353-358，2000.

5. Gao J-T, Tang H-R, Zhao B-L: The ESR study on the protective effect of grape seed extract on rat heart mitochondria from injury of lipid peroxidation. Res Chem Intermed 26,817-828 2000.

6. Tang H-R, Zhao B-L, Belton P S, Sutchiffe L H, Ng A: Anomalous proton NMR relaxation behavior of cell wall materials from Chinese water chesnut. Magn Reson Chem 38,765-770,2000.

7. Zhao B-L, Zhou W-A, Ni,Y-C, Hou,J-W, Gao,T-L, Xin,W-J: Kinetic scavenging effects of chinonin on NO and oxygen free radicals generated from ischemia reperfusion myocardium and its protection effects on the myocardium. Res Chem Intermed 747-762,2000.

8. Zhao B-L, Guo Q, Xin W-J: Free radical scavenging by green tea polyphenols. Method Enzym 335 217-231,2001

9.  Nie G-J, Wei T-T, Zhao B-L:Polyphenol protection of DNA against damage. Method Enzym 335 232-231244,2001

10. Gao J-T, Tang H-R, Zhao B-L: The toxicological damagement of gas phase cigarette smoke on cells and protective effect of green tea polyphenols. Res Chem Intermed.27,269-280,2001.

11. Zhang D-L, Xiong J, Hu J,Li Y, Zhao B-L: Improved method to detect nitric oxide in biological syste. Appl Magn Reson 20,345-358,2001.

12. Nie GJ, Jin C-F, Cao Y-L, Shen S-R, Zhao B-L: Distinct effects of tea catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells. Arch Biochem Biophys 397,84-90,2002.

13. Nie GJ, Cao YL, Zhao B-L: Protective effects of green tea polyphenols and their major component, (-)-epigallocatechin-3-gallate (EGCG), on 6-hydroxyldopamine-induced apoptosis in PC12 cells. Redox Report,7,170-177,2202.

14. Gao JT, Tang HR, Li Y, Liu H, Zhao B-L: EPR study of the toxicological effects of Gas-phase cigarette smoke and the protective effects of grape seed extract on the mitochondrial membrane. Applied Magnetic Resonance 22,497-511,2002.

15. Zhang Y-T, Zhang D-L, Cao Y-L, Zhao B-L: Developmental experession and activity variation of nitric oxide synthesase in the brain of golden hamster. Brain Res Bulletin 58,385-389,2002.

16. Chen L-J, Yang X-Q, Jiao H-L, Zhao B-L: Tea catechins protect against Lead-induced cytotoxicity, lipid peroxidation, and membrane fluidity in HepG2 Cells. Toxcol Sci 69,149-156,2002.

17. Guangyin Zhou,  Wen Jiang,  Yan Zhao, Guangen Ma，Wenjuan Xin,  Junjie Yin and Baolu Zhao.Interaction between sodium tanshinone IIA sulfonate and the adriamycin semiquinone free radical: A possible mechanism for antagonizing adriamycin-induced cardiotoxity. Res Chem Interm. 28,277-290,2002.

18. Li W, Wei T-T, Xin W-J, Zhao B-L: Green tea polyphenols in combination with copper(II) induce apoptosis in Hela cells. Res Chem Interm. 28,505-618,2002.

19. Jiao H-L, Zhao B-L. Cytotoxic effect of peroxisome proliferator fenofibrate on human HepG2 hepatoma cell line and relevant mechanisms. Toxcol Appl Pharm 185,172-179,2002.

20. Cao Y-L, Niu Y-C, Zhao B-L: Study of effect of NO on wheat stripe rust by ESR. Free Rad Biol Med, 33 ,S74,2002.

21.  Shen J-G, Fung PCW, Xin W-J, Zhao B-L: Nitric oxide contributes to restribution of phosphatidylserine and triggers apoptosis via peroxynitrite and p53 pathway in hypoxia-reoxygenated cardiomyocyte. Free Rad Biol Med, 33 ,S82,2002.

22. Fung PCW, Shen J-G, Xin W-J, Zhao B-L: Gikgo Biloba extract (EGB 761) against  hypoxia-reoxygenated induced cardiomyocyte apoptosis via inhibiting NO/ONOO pathway and adjusting bcl-2,p53 and p21/wal/cip 1 expression_. Free Rad Biol Med, 33 ,S82-83,2002.

23. Nie G-J, Jin C-F, Shen S-R, Zhao B-L: Effect of green tea polyphenols on 6-HODA-induced apoptosis in PC12 cells. Free Rad Biol Med, 33 ,S212,2002.

24. Zhang D-L, Tao Y, Duan S-J, Rohdwald P, Zhao B-L: Pycnogenol in Cigarette Filters Scavenges Free Radicals and Reduces Mutagenicity and Toxicity of Tobacco Smoke In Vivo. Toxicol Indus Health 18,215-224, 2002.

25. Liu Q, Tao Y, Zhao B-L: ESR study on scavenging effect of nicotine on free radicals. Appl Mag Reson. 24,105-112,2003.

26. Zhou G-Y, Jiang w, Zhao Y, Ma G-E, Xin W-J, Yin J, Zhao B-L:Sodium tanshinone IIA sulfonate mediates electron transfer reaction in rat heart mitochondria. Biochem Pharmacol. 65，51-57，2003。

27. Zhao-BL，Antioxidant effects of green tea polyphenols。CHINESE SCI BUL.  48 (4) : 315-319，2003。

28. Shen J-G, Qiu X-S, Jiang B, Zhang D-L, Xin W-J, Fung P.C.W., Zhao B-L: Nitric oxide and oxygen radicals induced apoptosis via bcl-2 and p53 pathway in hypoxia-reoxygenated cardiomyocytes. Sci China. 46,27-39,2003.

29. Deliang Zhang, Zhiyv Niu, Qian Wan, Baolu Zhao： Stability and Reaction of Dithiocarbamate-ferrous-NO Complex in PMA-stimulated Peritoneal Macrophages。Res Chem Interm. 29,201-212,2003.

30.  Zhang Yueting and Zhao Baolu ：Green tea polyphenols enhance sodium nitropru

sside induced neurotoxicity in human neuroblastoma SH-SY5Y cells. J Neur Chem.86,1189-1200,2003.31. Jiao H-L, Ye P, Zhao B-L: Protective effects of green tea polyphenols on human HepG2 cells against oxidative damage of fenofibrate. Free rad Biol Med 35(9)，1121-1128，2003.

32. Chen L-J, Yang X-Q, Jiao H-L, Zhao B-L: Tea Catechins protect against lead-induced ROS formation, mitochondrial dysfunction and calcium dysregulation in PC 12 cells. Chem Res Toxiol 16,1155-1161,2003.

33. Xu Y-C, Zhao B-L: The main origin of endogenous NO in higher non-leguminous plant. Plant Bioph Biochem 41，833－838， 2003.

34. Haiyan Zeng, Qi Chen, Baolu Zhao: Genistein ameliorated β-amyloid peptide -induced hippocampal neuronal apoptosis. Free Rad Biol Med 36,180-188,2004.

35. Liu Q and Zhao B-L: Nicotine attenuates β-amyloid peptide induced neurotoxicity, free radical and calcium accumulation in hippocampal neuronal cultures. Brit J. Pharmocol 141，746-754，2004。

36. Guo P, Cao Y-L, Li Z-Q, Zhao B-L: Role of an endogenous nitric oxide burst in the resistance of wheat to stripe rust. Plant Cell Environ. 27 (4), 473-477，2004。

37. Xu Y-C, Cao Y-L, Guo P, Zhao B-L: Detection of nitric oxide in plants by electron spin resonance. Phytopath 94,402-407,2004.

38. Zhang D-L, Yin J-J，Zhao B-L: Oral administration of Crataegus extraction protects against ischemia/reperfusion brain damage in the Mongolian gerbils. J Neur Chem.. 90，211-219, 2004.

39. Chen,LJ Yang XQi, Jiao HL, Zhao BL：Effect of Tea Catechins on the Change of Glutathione Levels Caused by Pb++ in PC12 Cells. Chem Res Toxiol 17，922-928，2004.

40. Zhang J, Wang X-F, Lu Z-B, Liu N-Q, Zhao B-L: The effects of Meso 2, 3-dimercaptosuccinic acid and Olipomeric Procyanidins on acute lead neurotoxicity in rat hippocampus. Free Rad Biol Med 37，1037-1050，2004。

41. Yueting Zhang Jie Zhang and Baolu Zhao：Nitric oxide synthase inhibition prevents neuronal death in the developing visual cortex. Eur J Neurosci 20,2251-2259,2004.

42. Liu HL, Zhang DL, Zhao BL, Zhao JY：Superoxide anion, the main species of ROS in the development of ARDS induced by oleic acid。FREE RADICAL RES 38: 1281-1287，2004。

43. Baolu Zhao: Nitric oxide in neirodegenarative diseases. Front Biosci 10, 454-461, 2005.

44. Baolu Zhao: Natural antioxidant for neurodegenerative diseases. Mol Neurobiol, 31, 283-293，2005

45. Cao Y, Guo P, Zhao B-L Simultaneous detection of NO and ROS by ESR in biological system. Method Enzymol  396,77-83, 2005

46. Xu Y-C, Cao Y-L, Tao Y, Zhao B-L: Technique of detection of NO in plants by ESR spin trapping. Method Enzym  396,84-92, 2005

47. S-H Guo, E Bezard, B-L Zhao：Protective effect of green tea polyphenols on the SH-SY5Y cells against 6-OHDA induced apoptosis through ROS-NO pathway. Free Rad Biol Med 39: 682-695，2005;

48. Yu-Xiang Xie, Erwan Bezard, Bao-Lu. Zhao: Unraveling the receptor- independent neuroprotective mechanism in mitochondria. J. Biol Chem. 37，32405-32412，2005。

49. Astrid Gutierrez-Zepeda, Ross Santell, Zhixin Wu, Marishka Brown, YanJue Wu, Ikhlas Khan, Christopher D Link, Baolu Zhao and Yuan Luo：Soy isoflavone glycitein protects against beta amyloid-induced toxicity and oxidative stress in transgenic Caenorhabditis elegans.BMC Neuroscience. 2005, 6:54，1-9。

50. Wu Ke, Huang Changgang, Cao Yuanlin，Zheng Yingguang，Cong Jianbo, Xian Hong, Wang Changzhen, Gao Shangkai ,Zhao Baolu. Plate form three-dimensional gradient coils for L-band ESR imaging experiment” published in the Journal of Magnetic Resonance, 2005; 175: 256-263.

51. Zhongbin Lu, Guangjun Nie, Peter Belton, Huiru Tang, Baolu Zhao. Structure-activity Relationship Analysis of Antioxidant Ability and Neuroprotective Effect of Gallic Acid Derivatives. Neurochem Int 48,263-274 2006.

52. Baolu Zhao. The health effects of tea polyphenols and their antioxidant mechanism. J. Clinical Biochem. Nutrition, 38 (2006).59-68.

53. Jie Zhang, Qiang Liu Nian-Qing Liu, Fu-Liang Li, Qi Chen, Chuan Qin, Hua Zhu， Yu-Ying Huang, Wei He, Bao-Lu Zhao.Nicotine reduces β-amyloidosis by regulating metal homeostasis. FASEB J 20，1212-1214，2006。

54. Zhang Jie, Mori, Akitane, Zhao Baolu.Fermented papaya preparation attenuates APP:A mediated copper neurotoxicity in APP and APPsw overexpressing SH-SY5Y cells。Neuroscience 143 ，63–72，2006。

55. Zhongbing Lu, Yi Tao, Zhixiang Zhou, Junjing Zhang, Cong Li, Lingcheng OU, Baolu Zhao. Mitochondrial ROS and NO Mediated Cancer Cell Apoptosis in 2-BA-2-DMHB Photodynamic Therapy. Free Rad Biol. Med. 41，1590–1605，2006。

56. Qiang Liu, Jie Zhang, Hua Zhu, Chuan Qin, Qi Chen，Baolu Zhao. Dissecting the Signalling Pathway of Nicotine-Mediated Neuroprotection in a Mouse Alzheimer Disease Model. FASEB J (in press)

57. Guoliang Jie, Zhi Lin, Longze Zhang, Haipeng Lv,  Puming He, Baolu Zhao。Free radical scavenging effect of Pu-erh tea extracts and their protective effect on the oxidative damage in the HPF-1 cells。J Agricu Food Chem （in press，2006）

58.  Baolu Zhao, Gengtao Liu, Lester Packer: Natural antioxidants and micronutrients. Medimond, Editografica. Rastignano.Bologna (Intaly), 2005.

59.  赵保路: 专著“氧自由基和天然抗氧化剂”科学出版社 2002（再版）

专利

1．低自由基低毒卷烟及其生产方法(申请号96116865、7)1995年申请；2001授权批准（专利号95116865.7）

2．一种有效清除卷烟烟气中自由基的卷烟滤嘴及其制备方法：(申请号00130133.0),2000年申请，2003年授权批准（专利号ZL00130133.0）。

3．国际专利Cigarette filter with scavenging effect on free radicals in cigarette smoke and its preparation method，2004 年授权批准（专利号US 6,832,612,B2）;

4．一种抑制肿瘤生长、清除自由基和抗脂质过氧化的动物软骨胶囊及其制备方法。1999年申请，2003年授权批准（专利号ZL9910076.0）。

5．一种抗老年痴呆症复合制剂：(申请号200410042774。7),2004年。

6．一种抗帕金森症复合制剂：(申请号200410047571。7),2004年。

国际会议特邀报告：

1. “Free radical reaction of green tea polyphenols” In 2000 4th UNESCO-MCBN/COSTAM/SFFR (ASEAN) WORKSHOP Micronutrients and Health: Molecular Biological Mechanisms as invited speaker. Malaysia.

2. “The scavenging effect of antioxidant on NO free radical in ischemia-reperfusion heart and their protective effect” In 2001 The Second International Symposium on Natural Antioxidants. Beijing, China. 大会主席:刘耕陶。

3. “ESR studies of NO free radicals generated from biological system.” In 2001 EPR Visible Systems 9th International Meeting. USA.

4. “Preventive Effects of Green Tea Polyphenols on 6-Hydroxydopamine-Induced Apoptosis in PC12 Cells” In 2001, 1th International Conference on Alzheimers Disease and Related Neurodegenerative Disorders. Wuhan,China.

5. “ESR spin trapping study on NO free radicals generated from wheat stripe rust.” 7th International Symposium on Spin Trapping. Chapel Hill, NC,USA. 2002.

6. Oral administration of Crataegus extraction protects against ischemia/reperfusion brain damage in the Mongolian gerbils. 8th International Symposium on Spin Trapping. Kyushu, Japan 2003。大会主席: Utsumi Hideo.

7．Anti-apoptotic effect of green tea polyphenols on neurons. 4th East Asia Biophysics Symposium. Taipei, 2003.

8. Smoke free radicals and health, scavenging effects of pycnogenol on free radicals generated from cigarette smoke and detoxification effects.. International Symposium on Free radical and Health. Sakata, Japan.2003. 大会主席：Hiramatsu Midori.

9. Antioxidants prevent neurodegenerative diseases. ISN Satellite Meeting:Oxidative Stress in Neurodegenerative Disorder. Guilin China, 2004. 。

10. Genistein ameliorated β-amyloid peptide -induced hippocampal neuronal apoptosis. 9th International Conference on Alzheimers Disease and Related Disorders in Philadelphia, Pennsylvania, USA, July 17-22, 2004. 大会主席：Kerri Leo。

11.  自由基、抗氧化剂与衰老.第六届全国自由基生物学与自由基医学学术会议和海峡两岸自由基生物学与自由基医学学术会议。2004年9月17~21日，西安。

12. Protective Effects of Natural antioxidants on neurodegenration diseases. The Third International Symposium on Natural Antioxidants- Molecular Mechanisms and Health Effects (ISNA) and Meeting of the Society for Free Radical Research (SFRR Asia), Shanghai, China.Jun 24-29,2005.

13. Why drinking green tea prevents obesity? The 13th Sendai Conference on Lipid Peroxide Biology and Medicine. Sendai, Japan, August 15,2005. 。

14．自由基、抗氧化剂与衰老。中国科学技术协会第七届学术年会。2005年8月20-23日在新疆乌鲁木齐市。

15. “Development of L-band and X-band ESR imaging systems in China” International Conference on Electron Paramagnetic Resonance Spectroscopy and Imaging of Biological Systems – (EPR 2005). Colunbus, Ohio USA. September 4-8, 2005.大会主席KUPPUSAMY, PERIANNAN

16. Protective effects of green tea polyphenols against 6-OHDA induced apoptosis throng ROS-NO pathway in parkinson’s disease. Collegium International Neuro-Phychopharmacolocium (CINP) Asia Pacific Regional Meeting. 14-17/3/2006, Pattaya, Thailand.

17. 自由基、抗氧化剂与衰老. 第十次中国生物物理大会暨第八届会员代表大会. 2006年5月23-27日在青岛市.

18. Effects of nicotine on Alzheimer’s disease and Parkinson’s disease. 1st Shanghai Symposium on Tobacco and Health Jun 8-9, 2006, Shanghai, China.

19. The Anti-obesity Effect of Green Tea Polyphenols through a Noval Mechanism of Lipids Oxidation and Antioxidant. 13thBiennial Meeting of the Society for Free Radical Research International. !5-19/8/2006, Davos, Switzerland.