王德寿,男,四川广安人,1964年9月生 理学博士,教授,博士导师,重庆市水产学学术技术带头人,淡水鱼类资源与生殖发育教育部重点实验室主任。
先后在PLoS Genetics、Molecular Ecology Resources、Genetics、Endocrinology等杂志发表学术论文160余篇,120余篇论文被SCI收录,h-index达到31,被SCI论文引用3500余次。任Comparative Biochemistry and Physiology - Part B编委。多次在国际学术会议上作特邀报告或大会发言。获得发明专利5项。
教育及工作经历:
1981.09-1988.07 西南师范大学学士、硕士
1993.09-1996.07 中山大学博士
1988.06-至今 西南(师范)大学副教授(1993)、教授(1998)、博导(2003)
1998.07-1999.08 以色列Bar-Iran大学博后
1999.09-2000.09 荷兰乌德勒支大学访问学者
2001.06-2001.09 香港中文大学高级访问学者
2001.11-2007.10 日本国立基础生物学研究所合作研究
2009.01-2011.08 任淡水鱼类资源与生殖发育教育部重点实验室副主任
2011.09-至今 任淡水鱼类资源与生殖发育教育部重点实验室主任
2009.01-至今 任重庆市发改委淡水鱼类新品种繁育工程实验室主任。
2011.12-2019.3 西南大学生命科学学院院长。
学术兼职:
1、国际鱼类内分泌学会理事(International Society for Fish Endocrinology Commission Member)。
2、中国鱼类学会副理事长。
3、中国水产学会水产生物技术专业委员会委员。
4、中国动物学会比较内分泌学分会理事。
5、中国遗传学会动物遗传专业委员会委员。
6、重庆动物学会理事长。
主讲课程:
主讲《动物学》、《分子生物学》、《鱼类遗传育种》等课程。
研究方向:
主要从事鱼类生理学、分子生物学和鱼类基因组学方面的研究工作。
研究领域:
鱼类性别决定与分化和生长调控的分子机制。
承担科研项目情况:
先后主持包括国家重点研发计划(蓝色粮仓)课题、国家自然科学基金重点项目、中美国际合作项目、面上项目等国家和省市课题20余项。
1. 科技部蓝色粮仓重点研发计划课题:水产养殖动物性别决定与发育的调控机制。
2. 中美(NSFC-NSF)生物多样性合作研究项目:性染色体:一个尚未被探究的生物多样性维度。
3. 国家自然科学基金重点基金:Amhy决定尼罗罗非鱼性别的分子机制研究。
4. 国家自然科学基金面上基金:罗非鱼体色突变体的遗传解析。
5. 重庆市科技局“重庆英才计划”:鱼类性别决定与性控育种团队创新创业示范团队。
认定成果:
1 人工诱导分化卵巢去分化再分化模型研究 王德寿;周林燕;张耀光;魏静;王志坚;吴凤瑞;蒲德永;黄宝锋;孙运侣 西南大学 2015
2 尼罗罗非鱼性染色体特异分子标记及遗传性别鉴定方法 王德寿;孙运侣;曾圣;叶凯;黄宝锋 西南大学 2013
3 罗非鱼性别分化的分子机制研究:转录因子对芳香化酶的调控作用 王德寿;周林燕;黄希贵;刘智皓;胡重江;吴风瑞;张未丽;韩飞 西南大学 2010
4 南方鲇全雌化人工繁殖技术研究 王德寿;张耀光;王志坚 西南大学 2006
发明公开:
[1]魏静, 张婷婷, 王德寿. 一种源于罗非鱼抗菌肽piscidin 4的人工改造肽及其应用[P]. 重庆市: CN119019524A, 2024-11-26.
[2]陶文静, 王德寿, 于淼, 孙丽娜, 李明辉. 一种cpeb1基因在调控动物生殖能力中的应用[P]. 重庆市: CN118638800A, 2024-09-13.
[3]王德寿, 孙丽娜, 李志强, 覃祖亮, 高田, 李明辉, 陶文静. 采用基因编辑技术创制粉色大口黑鲈的方法[P]. 重庆市: CN117844816A, 2024-04-09.
[4]王德寿, 孙丽娜, 杨双溢, 陶文静, 李明辉. sstr2a基因敲除尼罗罗非鱼突变体及其制备方法和应用[P]. 重庆市: CN117778392A, 2024-03-29.
[5]魏静, 李佳能, 王德寿. 一种尼罗罗非鱼精巢间质细胞系的建立方法[P]. 重庆市: CN117778297A, 2024-03-29.
[6]王德寿, 李明辉, 邹远超, 郑树清, 戴生飞, 韩林强, 粱建辉. 一种白乌鳢X染色体特异的分子标记及其应用[P]. 重庆市: CN117778590A, 2024-03-29.
[7]王德寿, 王晨旭, 陶文静, 李明辉, 柳兴永, 郑树清, 孙丽娜, 魏静, 魏玲. 一种兼具观赏和食用价值的肉红色罗非鱼生产方法[P]. 重庆市: CN117327740A, 2024-01-02.
[8]王德寿, 陆宝悦, 梁广源, 王晨旭, 柳兴永, 孙丽娜, 李明辉, 陶文静. 一种无色透明罗非鱼的生产方法[P]. 重庆市: CN117305369A, 2023-12-29.
[9]王德寿, 徐嘉, 梁广源, 李鹏, 王晨旭, 吴晋芝, 柳兴永, 孙丽娜, 李明辉, 陶文静. 控制鱼类红色素细胞谱系分化和形成的关键基因pax3及其应用[P]. 重庆市: CN117305370A, 2023-12-29.
[10]王德寿, 梁广源, 陆宝悦, 王晨旭, 柳兴永, 孙丽娜, 李明辉, 陶文静. 一种白色罗非鱼的生产方法[P]. 重庆市: CN117286187A, 2023-12-26.
[11]王德寿, 陆宝悦, 梁广源, 王晨旭, 柳兴永, 孙丽娜, 李明辉, 陶文静. 一种银白色罗非鱼的生产方法[P]. 重庆市: CN117265018A, 2023-12-22.
[12]王德寿, 梁广源, 陆宝悦, 药嘉文, 王晨旭, 柳兴永, 孙丽娜, 李明辉, 陶文静, 郑树清. 一种调控鱼类红色素的基因pax7序列、方法及应用[P]. 重庆市: CN117126857A, 2023-11-28.
[13]王德寿, 陶文静, 朱喜, 孙丽娜, 李明辉. 一种齐氏罗非鱼性染色体特异分子标记、检测引物、试剂盒及其用途[P]. 重庆市: CN116804225A, 2023-09-26.
[14]王德寿, 郑树清, 陶红艳, 杨浩文. 鉴定南方鲇、鲇及其杂交种的特异分子标记、引物和试剂盒[P]. 重庆市: CN116574817A, 2023-08-11.
[15]王德寿, 郑树清, 杨浩文, 陶红艳. 鲇性别鉴定的特异分子标记、引物组、试剂盒、方法及应用[P]. 重庆市: CN116555410A, 2023-08-08.
[16]王德寿, 李明辉, 邹远超, 郑树清, 周朝伟, 苏建. 一种乌鳢性别特异分子标记、基于该分子标记的遗传性别鉴定方法和应用[P]. 重庆市: CN115725746A, 2023-03-03.
[17]魏静, 张泽明, 王德寿, 白孝明. 一种高效的日本青鱂体外培养细胞CRISPR/Cas9基因组编辑方法及其应用[P]. 重庆市: CN115521899A, 2022-12-27.
[18]王德寿, 陆宝悦, 王晨旭, 谭德阶, 陶文静, 孙丽娜, 李明辉, 柳兴永. 全雄无黑色素红罗非鱼的生产方法[P]. 重庆市: CN113875655A, 2022-01-04.
[19]谷浪屿, 李陈峥, 毛晓兵, 韦宗仿, 黄友葵, 何希敏, 周文君, 李礼, 王德寿. 核酸序列及其作为启动子的应用[P]. 广东省: CN112553196A, 2021-03-26.
[20]王德寿, 赵琳, 陶文静, 伍鑫, 樊征. 奥利亚罗非鱼性染色体特异分子标记及基于该分子标记的遗传性别鉴定和单性鱼生产方法[P]. 重庆市: CN110938626A, 2020-03-31.
[21]王德寿, 柳兴永, 李明辉, 陆宝悦, 蒋小龙, 石红娟. 一种高效生产尼罗罗非鱼高繁殖率YY转化雌鱼的方法[P]. 重庆市: CN110583539A, 2019-12-20.
[22]王德寿, 郑树清, 王晓双, 张帅, 龙娟. 南方鲇性染色体特异分子标记与基于该分子标记的遗传性别鉴定方法和单性鱼生产方法[P]. 重庆市: CN110551808A, 2019-12-10.
[23]江东能, 黄远青, 王德寿, 孙运侣, 李明辉, 李广丽, 司徒家欣, 邝紫颖, 谢恩义, 石红娟. 一种快速培育YY超雄尼罗罗非鱼的方法[P]. 广东省: CN110250116A, 2019-09-20.
[24]周林燕, 方雪莲, 罗凤, 杨兰英, 王德寿. 罗非鱼pgr/mstn基因纯合敲除雌性完全不育品系及构建方法[P]. 重庆: CN108624619A, 2018-10-09.
[25]王德寿, 孙运侣, 江东能, 黄宝锋, 曾圣, 叶凯. 尼罗罗非鱼Y染色体特异分子标记与基于该分子标记的遗传性别鉴定方法和超雄鱼生产方法[P]. 重庆: CN103409420A, 2013-11-27.
[26]王德寿, 孙运侣, 江东能, 黄宝锋, 曾圣, 叶凯. 尼罗罗非鱼X染色体特异分子标记[P]. 重庆: CN103409421A, 2013-11-27.
[27]王德寿, 孙运侣, 曾圣, 叶凯, 黄宝锋. 尼罗罗非鱼性染色体特异分子标记[P]. 重庆: CN102533751A, 2012-07-04.
[28]王德寿, 王黔, 黄宝锋, 孙运侣. 一种简易高效的循环水孵化系统[P]. 重庆: CN102177861A, 2011-09-14.
[29]王德寿, 王志坚, 张耀光, 周成果, 匡刚桥, 孙运侣. 高效经济的室内循环水养殖系统[P]. 重庆: CN102177867A, 2011-09-14.
[30]王德寿, 黄宝锋, 孙运侣, 刘智皓, 杨世杰. 法倔唑在诱导罗非鱼已分化卵巢转变为功能型精巢中的应用及其诱导方法[P]. 重庆: CN102068429A, 2011-05-25.
[31]王德寿, 孙运侣, 曾圣, 叶凯, 黄宝锋. 尼罗罗非鱼性染色体特异分子标记及遗传性别鉴定方法[P]. 重庆: CN101962641A, 2011-02-02.
发明授权:
[1]王德寿, 陶文静, 朱喜, 孙丽娜, 李明辉. 一种齐氏罗非鱼性染色体特异分子标记、检测引物、试剂盒及其用途[P]. 重庆市: CN116804225B, 2024-02-13.
[2]王德寿, 赵琳, 陶文静, 伍鑫, 樊征. 奥利亚罗非鱼性染色体特异分子标记及基于该分子标记的遗传性别鉴定和单性鱼生产方法[P]. 重庆市: CN110938626B, 2023-07-18.
[3]谷浪屿, 李陈峥, 毛晓兵, 韦宗仿, 黄友葵, 何希敏, 周文君, 李礼, 王德寿. 核酸序列及其作为启动子的应用[P]. 广东省: CN112553196B, 2021-08-10.
[4]张玉, 王德寿. 一种立式开槽机用便于更换的压板组件及其更换方法[P]. 安徽省: CN111673510B, 2021-07-27.
[5]王德寿, 郑树清, 王晓双, 张帅, 龙娟. 南方鲇性染色体特异分子标记与基于该分子标记的遗传性别鉴定方法和单性鱼生产方法[P]. 重庆市: CN110551808B, 2021-07-06.
[6]王德寿, 柳兴永, 李明辉, 陆宝悦, 蒋小龙, 石红娟. 一种高效生产尼罗罗非鱼高繁殖率YY转化雌鱼的方法[P]. 重庆市: CN110583539B, 2021-05-28.
[7]王德寿, 孙运侣, 江东能, 黄宝锋, 曾圣, 叶凯. 尼罗罗非鱼Y染色体特异分子标记与基于该分子标记的遗传性别鉴定方法和超雄鱼生产方法[P]. 重庆市: CN103409420B, 2015-04-15.
[8]王德寿, 孙运侣, 江东能, 黄宝锋, 曾圣, 叶凯. 尼罗罗非鱼X染色体特异分子标记[P]. 重庆市: CN103409421B, 2015-02-11.
[9]王德寿, 孙运侣, 曾圣, 叶凯, 黄宝锋. 尼罗罗非鱼性染色体特异分子标记及遗传性别鉴定方法[P]. 重庆市: CN101962641B, 2013-06-05.
[10]王德寿, 王黔, 黄宝锋, 孙运侣. 一种简易的循环水孵化系统[P]. 重庆市: CN102177861B, 2012-11-21.
[11]王德寿, 黄宝锋, 孙运侣, 刘智皓, 杨世杰. 法倔唑在诱导罗非鱼已分化卵巢转变为功能型精巢中的应用及其诱导方法[P]. 重庆市: CN102068429B, 2011-12-14.
参编专著
1. 何学福、张耀光主编,王德寿、洪锡钧、罗泉笙副主编。四川经济鲇类研究。西南师范大学出版社。1998年7月。
2. 陈松林主编,王德寿参编,鱼类基因组学及基因组育种技术。科学出版社。2017年5月
3. Deng, Si-Ping; Liu, Zhi-Hao; Wang, De-Shou. 2019. Mechanisms of Feminization and Sex Differentiation in Southern Catfish. Sex Control in Aquaculture. Edited by Han-Ping Wang, Francesc Piferrer, Song-Ling Chen. Vol. II, Pages: 509-524. Published by Wiley & Sons Ltd (Wiley Blackwell).
发表英文论文: *通讯作者
[1]Wu, You; Yang, Lanying; Du, Yiyun; Su, Yun; Zhao, Chenhua; Li, Lu; Cai, Jing; Wang, Deshou*; Zhou, Linyan*.New insights into the role of myostatin in fish fertility based on the findings in mstnb-deficient Nile tilapia (Oreochromis niloticus).Aquaculture Reports, 2024, 35: 101926.
[2]Xiang, Liu; Changle, Zhao; Lei, Liu; Xi, Peng; Jianeng, Li; Wenjing, Tao; Deshou, Wang; Jing, Wei.Nile Tilapia (Oreochromis niloticus) Patched1 Mutations Disrupt Cardiovascular Development and Vascular Integrity through Smoothened Signaling.International Journal of Molecular Sciences, 2024, 25(6): 3321.
[3]Wang, Chenxu; Kocher, Thomas D.; Liu, Hao; Wen, Zilong; Yao, Jiawen; Wang, Deshou*.Knockout of Hermansky-Pudlak syndrome 5 (hps5) leads to red tilapia with reduced melanophores and iridophores.Aquaculture, 2024, 582: 740496.
[4]Yu, Miao; Zhang, Shiyi; Ma, Zhisheng; Qiang, Jun; Wei, Jing; Sun, Lina; Kocher, Thomas D.; Wang, Deshou*; Tao, Wenjing*.Disruption of Zar1 leads to arrested oogenesis by regulating polyadenylation via Cpeb1 in tilapia (Oreochromis niloticus).International Journal of Biological Macromolecules, 2024, 260: 129632.
[5]Li, Minghui; Sun, Lina; Zhou, Linyan; Wang, Deshou*.Tilapia, a good model for studying reproductive endocrinology.General and Comparative Endocrinology, 2024, 345: 114395.
[6]Zhu, Song*; Miao, Bo; Zhang, Yu-Zhou; Yang, Qin; Deng, Xun-Teng; Gao, Li-Hong; Zheng, Ji-Shu; Pu, De-Cheng; Wang, De-Shou; Su, Sheng-Qi*.Establishment of a tilapia lake virus (TiLV)-susceptible cell line for anti-TiLV Chinese herbal compound screening.Aquaculture, 2023, 577: 739999.
[7]Zhao, Changle; Liu, Xiang; Liu, Lei; Li, Jianeng; Liu, Xingyong; Tao, Wenjing; Wang, Deshou*; Wei, Jing*.Smoothened mediates medaka spermatogonia proliferation via Gli1-Rgcc-Cdk1 axis.Biology of Reproduction, 2023, 109(5): 772-784.
[8]Zheng, Shuqing; Tao, Wenjing; Tao, Hongyan; Yang, Haowen; Wu, Limin; Shao, Feng; Wang, Zhijian; Jin, Li; Peng, Zuogang*; Wang, Deshou*; Zhang, Yaoguang*.Characterization of the male-specific region containing the candidate sex-determining gene in Amur catfish (Silurus asotus) using third-generation- and pool-sequencing data.International Journal of Biological Macromolecules, 2023, 248: 125908.
[9]Li, Xiao-Yan; Tang, Yao-Hao; Deng, Wan-Yue; Zheng, Yan; Wang, Ling-Song; He, Xue; Xie, Qing-Ping; Li, Yue-Qin; Deng, Li; Wang, De-Shou*; Wei, Ling*.Involvement of Sox9a in chondrogenesis and gonadal development in teleost Nile tilapia (Oreochromis niloticus).ZOOLOGICAL RESEARCH, 2023, 44(4): 729-731.
[10]Fan, Sen; Shi, Hong -Juan; Peng, You -Xing; Huang, Yuan-Qing; Jiang, Mou-Yan; Li, Guang-Li; Wang, De-Shou*; Jiang, Dong-Neng*.Dietary aromatase inhibitor treatment converts XY gsdf homozygous mutants to sub-fertile male in Nile tilapia (Oreochromis niloticus).Aquaculture, 2023, 569: 739381.
[11]He, Tao; Zhang, Yu-Zhou; Gao, Li-Hong; Miao, Bo; Zheng, Ji-Shu; Pu, De-Cheng; Zhang, Qing-Qing; Zeng, Wei-Wei; Wang, De-Shou; Su, Sheng-Qi*; Zhu, Song*.Identification and pathogenetic study of tilapia lake virus (TiLV) isolated from naturally diseased tilapia.Aquaculture, 2023, 565: 739166.
[12]Wang, Chenxu; Kocher, Thomas D.; Wu, Jinzhi; Li, Peng; Liang, Guangyuan; Lu, Baoyue; Xu, Jia; Chen, Xiaoke; Wang, Deshou*.Knockout of microphthalmia-associated transcription factor (mitf) confers a red and yellow tilapia with few pigmented melanophores.Aquaculture, 2023, 565: 739151.
[13]Bai, Xiaoming; Jianeng, Li; Zhang, Zeming; Qu, Ximei; Tao, Wenjing; Zhou, Linyan; Wang, Deshou*; Wei, Jing*.Nile tilapia (Oreochromis niloticus) Nanog co-expression with Pou5f3, transcriptional regulation and biological activity in embyonic development and embryonic cells.Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology, 2023, 264: 110812.
[14]Zhang, Zeming; Wang, Jie; Li, Jianeng; Liu, Xiang; Liu, Lei; Zhao, Changle; Tao, Wenjing; Wang, Deshou*; Wei, Jing*.Establishment of an Integrated CRISPR/Cas9 Plasmid System for Simple and Efficient Genome Editing in Medaka In Vitro and In Vivo.Biology-Basel, 2023, 12(2): 336.
[15]Liu, Xingyong; Zhou, Li; Li, Wenbo; Wu, Jiahong; Wang, Deshou*.Gata2a Mutation Causes Progressive Microphthalmia and Blindness in Nile Tilapia.International Journal of Molecular Sciences, 2023, 24(4): 3567.
[16]Wu, You; Wu, Tengfei; Yang, Lanying; Su, Yun; Zhao, Chenhua; Li, Lu; Cai, Jing; Dai, Xiangyan*; Wang, Deshou*; Zhou, Linyan*.Generation of fast growth Nile tilapia (Oreochromis niloticus) by myostatin gene mutation.Aquaculture, 2023, 562: 738762.
[17]Li, Minghui*; Liu, Xingyong; Lu, Baoyue; Sun, Lina; Wang, Deshou*.Sexual plasticity is affected by sex chromosome karyotype and copy number of sex determiner in tilapia.Aquaculture, 2022, 561: 738664.
[18]Li, Yueqin; Tang, Yaohao; Wang, Lingsong; Li, Xiaoyan; Deng, Li; Deng, Wanyue; Zheng, Yan; Wang, Deshou*; Wei, Ling*.Transcription factor Sox3 is required for oogenesis in the teleost fish Nile tilapia.International Journal of Biological Macromolecules, 2022, 222: 2639-2647.
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[102]Wu Limin; Yang Peng; Luo Feng; Wang Deshou; Zhou Linyan* R-spondin1 signaling pathway is required for both the ovarian and testicular development in a teleost, Nile tilapia (Oreochromis niloticus). General and Comparative Endocrinology, 2016, 230: 177-185.(IF: 2.47)
[103]Xie QP, He X, Sui YN, Chen LL, Sun LN,Wang DS*.Haploinsufficiency of Sf-1 causes fem ale to male sex reversal in Nile tilapia,Oreochromis niloticus. Endocrinology. 2016,157(6):2500-2514. doi: 10.1210/en.2015-2049. Epub 2016 Apr 5.(IF: 4.503)
[104]Tao W, Sun L, Shi H, Cheng Y, Jiang D, Fu B, Conte MA, Gammerdinger WJ, Kocher TD,Wang DS*.Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation. BMC Genomics. 2016,17(1):328. doi: 10.1186/s12864-016-2636-z.(IF: 3.867)
[105]Jiang DN, Yang HH, Li MH, Shi HJ, Zhang XB,Wang DS*. gsdfis a downstream gene of dmrt1 that functions in the male sex determination pathway of the Nile tilapia. Mol Reprod Dev. 2016,83(6):497-508. doi: 10.1002/mrd.22642. Epub 2016 May 1.(IF: 2.527)
[106]Wu L, Wu F, Xie L,Wang DS*, Zhou L*. Synergistic role of β-catenin1 and 2 in ovarian differentiation and maintenance of female pathway in Nile tilapia. Biochemical and Biophysical Research Communications, 2016, 473(1): 29-34. doi: 10.1016/j.mce.2016.03.002(IF: 3.859)
[107]Zhou L, Luo F, Fang X, Charkraborty T, Wu L, Wei J,Wang DS*. Blockage of progestin physiology disrupts ovarian differentiation in XX Nile tilapia (Oreochromis niloticus). Biochem Biophys Res Commun. 2016,473(1):29-34.(IF: 2.297)
[108]Chen L, Jiang X, Feng H, Shi H, Sun L, Tao W, Xie Q,Wang DS*. Simultaneous exposure to estrogen and androgen resulted in feminization and endocrine disruption. J Endocrinol. 2016,228(3):205-218.(IF: 4.498)
[109]Wei L, Yang C, Tao W,Wang DS*. Genome-wide identification and transcriptome-based expression profiling of the sox gene family in the Nile tilapia (Oreochromis niloticus). Int J Mol Sci.2016, 17(3):270. pii: E270. doi: 10.3390/ijms17030270.(IF: 2.862)
[110]Li M, Feng R, Ma H, Dong R, Liu Z, Jiang W, Tao W,Wang DS*.Retinoic acid triggers meiosis initiation via stra8-dependent pathway in Southern catfish,Silurus meridionalis. General and Comparative Endocrinology,2016, 232:191-198. doi: 10.1016/j.ygcen.2016.01.003. Epub 2016 Jan 4.(IF: 2.47)
[111]Li MH, Sun YL, Zhao JE, Shi HJ, Zeng S, Ye K, Jiang DN, Zhou LY, Sun LN, Tao WJ, Nagahama Y, Kocher TD,Wang DS*.A tandem duplicate of anti-Müllerian hormone with a missense SNP on the Y chromosome is essential for male sex determination in Nile tilapia,Oreochromis niloticus. PLoS Genetics. 2015, 11(11): e1005678. doi:10.1371/journal.pgen.1005678.(IF: 6.661)
[112]Feng R, Fang L, Cheng Y, He X, Jiang W, Dong R, Shi H, Jiang D, Sun L,Wang DS*. Retinoic acid homeostasis through aldh1a2 and cyp26a1 mediates meiotic entry in Nile tilapia (Oreochromis niloticus). Scientific Reports, 2015, 15(5):10131. doi: 10.1038/srep10131.(IF: 5.578)
[113]Qiu YX; Sun SH; Charkraborty T; Wu LM; Sun LN; Wei J; Nagahama Y; Wang DS*; Zhou LY* Figla Favors Ovarian Differentiation by Antagonizing Spermatogenesis in a Teleosts, Nile Tilapia(Oreochromis niloticus). PLoS One, 2015, 10(4):e0123900.(IF: 3.234)
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[116]Senthilkumaran B, Sreenivasulu G,Wang DS, Sudhakumari CC, Kobayashi T, Nagahama Y. 2015. Expression Patterns of CREBs in Oocyte Growth and Maturation of Fish. PLoS One, 2015, 10(12):e0145182. doi: 10.1371/ journal.pone.0145182. eCollection 2015.(IF: 3.234)
[117]Su Ting; Ijiri Shigeho*; Kanbara Hirokazu; Hagihara Seishi; Wang De Shou; Adachi Shinji.Characterization and expression of cDNAs encoding P450c17-II (cyp17a2) in Japanese eel during induced ovarian development.General and Comparative Endocrinology, 2015, 221: 134-143.
[118]Zhang X, Wang H, Li M, Cheng Y, Jiang D, Sun L, Tao W, Zhou L, Wang Z, andWang DS*. Isolation of Doublesex- and Mab-3-Related Transcription Factor 6 and Its Involvement in Spermatogenesis in Tilapia.Biology of Reproduction. 2014,91(6):136-145.(IF: 3.318)
[119]Dong RR, Yang SJ, Feng RJ, Fang LL, Sun YL, Zhang YG, Xie XJ,Wang DS*. Complete feminization of catfish by feeding Limnodilus, an annelid worm collected in contaminated streams. Environmental Research, 2014, 133: 371-379.(IF: 4.373)
[120]Yu XG#; Wu LM#; Xie L; Yang SJ; Charkraborty T; Shi HJ; Wang DS; Zhou LY*. Characterization of two paralogous StAR genes in a teleost, Nile tilapia (Oreochromis niloticus). Mol Cell Endocrinol, 2014, 392(1-2): 152-162.(IF: 4.405)
[121]Liu G, Luo F, Song Q, Wu L, Qiu Y, Shi H,Wang DS*,Zhou L*. Blocking of progestin action disrupts spermatogenesis in Nile tilapia (Oreochromis niloticus).Journal of Molecular Endocrinology, 2014, 53(1): 57-70.(IF: 3.081)
[122]Wei J, Qi W, Zhou Y, Zhang X, Dong R, Zhou L,Wang DS*.Establishment and characterization of an ovarian cell line from Southern catfish (Silurus meridionalis). Fish Physiology and Biochemistry, 2014, 40(5): 1383-1391.(IF:1.622).
[123]Li J, Wu P, Liu Y,Wang DS, Cheng CH. Temporal and spatial expression of the four Igf ligands and two Igf type 1 receptors in zebrafish during early embryonic development. Gene Expr Patterns, 2014, 15(2): 104-111.(IF:1.380).
[124]Sun YL, Jiang DN, Zeng S, Hu CJ, Ye K, Yang C, Yang SJ, Li MH,Wang DS*. Screening and characterization of sex-linked DNA markers and markerassisted selection in the Nile tilapia (Oreochromis niloticus), Aquaculture,2014, 433:19-27.(IF:1.878)
[125]Li MH; Yang HH; Zhao JE; Fang LL; Shi HJ; Li MR; Sun YL; Zhang XB; Jiang DN; Zhou LY; Wang DS*. Efficient and heritable gene targeting in tilapia by CRISPR/Cas9. Genetics, 2014, 197(2): 591-599.(IF:5.963).
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[127]Yuan J, Tao W, Cheng Y, Huang B,Wang DS*. Genome-wide identification, phylogeny, and gonadal expression of fox genes in Nile tilapia,Oreochromis niloticus.Fish Physiology and Biochemistry,2014, 40(4): 1239-1252.(IF:1.622).
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[135]Li JZ; Chen WJ; Wang DS; Zhou LY; Sakai F; Guan GJ; Nagahama Y.GATA4 is Involved in the Gonadal Development and Maturation of the Teleost Fish Tilapia, Oreochromis niloticus.Journal of Reproduction and Development, 2012, 58(2): 237-242.
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[137]Liu XH; Xie BW; Zhang YG; Wang DS; Wang ZJ*.cDNA cloning, pituitary location, and extra-pituitary expression of pro-opiomelanocortin gene in rare minnow (Gobiocypris rarus).Fish Physiology and Biochemistry, 2011, 37(1): 233-247.
[138]Li JZ, Liu ZH,Wang DS, Cheng CHK. Insulin-like growth factor 3 is involved in oocyte maturation in zebrafish. Biology of Reproduction, 2011, 84(3): 476-486.(IF:3.87)
[139]Han F, Wang ZJ, Wu FR,Liu ZH,Huang BF,Wang DS*. Characterization, phylogeny, alternative splicing and expression of Sox30 gene. BMC Molecular Biology, 2010, 11: 98.(IF:3.18)
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[144]Wu FR, Zhou LY, Nagahama Y,Wang DS*. Duplication and distinct expression patterns of two thrombospondin-1 isoforms in teleost fishes. Gene Expression Patterns, 2009, 9(6): 436-443.(IF:2.2)
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[151]Zhou LY,Wang DS,Kobayashi T, Yano A, Paul-Prasanth B, Suzuki A, Sakai F, Nagahama Y.A novel type of P450c17, lacking the lyase activity is responsible for C21-steroid biosynthesis in the fish ovary and head kidney. Endocrinology, 2007, 148(9): 4282-4291.(IF: 5.3)
[152]Huang XG,Jiao BW, Fung CK, Ho WKK, Zhang Y, Chan CB, Lin HR,Wang DS,Cheng CHK. The presence of two distinct prolactin receptors in seabream with different tissue distribution patterns, signal transduction pathways and regulation of gene expression by steroid hormones. Journal of Endocrinology, 2007, 194(2): 373-392.(IF:3.0)
[153]Liu ZH, Wu FR, Jiao BW, Zhang XY, Hu CJ, Huang BF, ZhouLY, Huang XG, Wang ZJ, Zhang YG, Nagahama Y, Cheng CHK,Wang DS*. Molecular cloning of doublesex and mab-3-related transcription factor 1, forkhead transcription factor gene 2, and two types of cytochrome P450 aromatase in Southern catfish and their possible roles in sex differentiation. Journal of Endocrinology, 2007, 194(1): 223-241.(IF:3.0)
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[8]龙娟; 郑树清; 王晓双; 张帅; 王德寿*.TGF-β信号通路在鱼类性别决定与分化中的作用.水产学报, 2020, 44(01): 166-177.
[9]魏静, 邓洪平, 罗克明, 陶文静, 周林燕, 孙丽娜, 魏玲, 李明辉, 王德寿. 高等院校“教师-研究生-本科生”联动创新实践平台的构建——以西南大学生命科学学院为例[J]. 西南师范大学学报(自然科学版), 2018, 43 (05): 180-184.
[10]王雅琴, 王德寿, 李英文, 刘智皓. 环境剂量的多种环境雌激素长期暴露阻断斑马鱼成鱼精子发生[J]. 重庆师范大学学报(自然科学版), 2018, 35 (01): 23-30.
[11]魏静, 于林田, 周林燕, 张小萍, 王德寿. 尼罗罗非鱼Foxp1a/b的cDNA克隆及表达模式研究[J]. 水产学报, 2015, 39 (12): 1763-1772.
[12]汪海, 王婷茹, 袁静, 杨超, 于祥国, 李明辉, 王德寿. 脊椎动物Dmrt基因的研究进展[J]. 贵州农业科学, 2012, 40 (05): 148-152.
[13]李明辉, 吴风瑞, 熊传奇, 曾圣, 杨世杰, 叶凯, 蒋汶洮, 孙运侣, 黄宝锋, 魏莹莹, 焦静, 顾源, 王德寿. 尼罗罗非鱼微阵列Fosmid基因组文库的构建及基因筛选[J]. 水产学报, 2011, 35 (01): 27-34.(通讯作者)
[14]张未丽, 刘智皓, 吴风瑞, 黄宝锋, 韩飞, 孙运侣, 王德寿. 转录因子GATA-2在脊椎动物发育过程中的作用[J]. 四川动物, 2009, 28 (06): 945-948.
[15]韩飞, 刘智皓, 吴风瑞, 黄宝锋, 张未丽, 周宏, 王德寿. 维甲酸和Cyp26基因家族与生殖细胞分化[J]. 细胞生物学杂志, 2009, 31 (03): 355-360.
[16]黄宝锋, 刘智皓, 吴风瑞, 王德寿*. 雄激素受体的结构和功能[J]. 四川动物, 2008, 27(02): 307-310.(通讯作者)
[17]张修月, 刘智皓, 王德寿*. 南方鲇受精细胞学观察[J]. 四川动物, 2008, 27(01): 7-11.(通讯作者)
[18]胡重江,吴风瑞,刘智皓,黄宝锋,张耀光,王德寿*(通讯作者). 南方鲇Vasa两种亚型cDNA的克隆及其表达研究. 动物学报.2008,54(6): 1051-1060.
[19]徐吉山, 王德寿, 张耀光. 大鳍鳠卵巢滤泡细胞发育的组织学研究[J]. 大理学院学报, 2007, (06): 10-14.
[20]章力, 黄希贵, 焦保卫, 吴风瑞, 王德寿*. 南方鲇两种生长激素受体的结构分析及其组织分布和激素调节[J]. 动物学报, 2006,52 (06): 1096-1106.(通讯作者)
[21]吴风瑞, 张修月, 王德寿*. 人工诱导南方鲇雌核发育的最适参数[J]. 动物学杂志, 2006, 41(01): 27-31.(通讯作者)
[22]刘智皓,张修月,王德寿*. 脊椎动物基因组与基因复制研究进展[J]. 四川动物, 2005, 24(04): 234-239.(通讯作者)
[23]章力,黄希贵,王德寿*. 鱼类胰岛素样生长因子(IGF)系统的研究进展[J]. 动物学杂志, 2005, 40(02): 99-105.(通讯作者)
[24]张修月,焦保卫,吴天利,金灿彪,王德寿. 南方鲶性腺分化的组织学观察[J]. 动物学杂志, 2005,40 (01): 41-48.
[25]邓思平,王德寿*,张耀光,焦保卫. 南方鲶生殖周期中脑垂体和血清促性腺激素水平的含量变化[J]. 西南师范大学学报(自然科学版), 2004,29 (04): 655-658.(通讯作者)
[26]黄希贵,焦保卫,王德寿*. 生长激素促分泌素受体(GHS-R)的发现及影响[J]. 动物学杂志, 2004,39 (02): 84-88.(通讯作者)
[27]周林燕 ,张修月 ,王德寿*. 脊椎动物性别决定和分化的分子机制研究进展[J]. 动物学研究, 2004,25 (01): 81-88.(通讯作者)
[28]邓思平,王德寿*,张耀光,吴天利. 外源激素对南方鲶血清促性腺激素水平的影响[J]. 西南师范大学学报(自然科学版), 2003, 28(04): 614-617.(通讯作者)
[29]邓思平,王德寿,张耀光,吴天利. 诱导南方鲶排卵过程中血清促性腺激素水平的变化[J]. 山地农业生物学报, 2003,22 (03): 214-217.
[30]周林燕,焦保卫,王德寿. 哺乳动物性别决定机理研究进展[J]. 海南大学学报(自然科学版), 2003, 21(01): 78-84.
[31]邓思平,王德*寿,张耀光,吴天利. LHRH-A和hCG对南方鲶血清促性腺激素水平的影响[J]. 湛江海洋大学学报, 2003, 23(01): 13-16.(通讯作者)
[32]陈康贵,王德寿,王瑞兰. 对胸鳍棘鉴定鱼类年龄方法的技术改进———简易脱钙切片法[J]. 动物学杂志, 2002, (05): 46-48.
[33]焦保卫,王德寿,邓思平. 爬行动物温度依赖性性别决定研究进展[J]. 动物学杂志, 2002, 35(04): 74-78.
[34]陈细香,金灿彪,徐吉山,王德寿. 大鳍鳠消化系统胚后发育的组织学研究[J]. 西南师范大学学报(自然科学版), 2002,27 (02): 239-243.
[35]王德寿,林浩然. 诱导大鳍鱯和长吻鮠排卵过程中血清GTH水平的变化[J]. 水生生物学报, 2001,25 (02): 185-190.
[36]邓思平,吴天利,王德寿,张耀光. 温度对南方鲶幼鱼生长与发育的影响[J]. 西南师范大学学报(自然科学版), 2000,25 (06): 674-679.
[37]王德寿,吴天利,张耀光. 鱼类性别决定及其机制的研究进展[J]. 西南师范大学学报(自然科学版), 2000,25 (03): 296-304.(通讯作者)
[38]王德寿,江宗秀. 鱼类性外激素的研究进展[J]. 水生生物学报, 2000,24 (03): 282-288.
[39]王德寿,林浩然,张为民. 大鳍鳠脑垂体和血清生长激素水平的季节变化[J]. 水产学报, 1999, 23(01): 1-5.
[40]王德寿,林浩然,H.J.Th.谷斯. 大鳍鱯促性腺激素分泌调控的研究[J]. 动物学报, 1998, 44(03): 75-81.
[41]王德寿,林浩然,蒲德永,张耀光,张其忠,何学福,蒋国福. 注射促黄体素释放激素类似物和地欧酮诱导大鳍鱯和长吻鮠排卵的研究[J]. 动物学研究, 1998, (03): 16-21.
[42]王德寿,林浩然,H.J.Th.Goos. 1997. 大鳍鱯脑垂体和血清促性腺激素(GTH)水平的季节变化. 鱼类学论文集(第六辑). 科学出版社(京), 第29-35页.
[43]张耀光,王德寿. 短尾拟鲿分类地位的探讨[J]. 水生生物学报, 1996,20 (04): 379-382.
[44]王德寿,林浩然. 鲶类生殖内分泌学研究[J]. 水产学报, 1996,20 (02): 151-158.
[45]张耀光,王德寿,蒲德永. 嘉陵江鲿科鱼类骨学研究(Ⅲ)─—头骨的比较[J]. 西南师范大学学报(自然科学版), 1995,20 (03): 284-292.
[46]张耀光,王德寿,蒲德永. 嘉陵江鲿科鱼类骨学研究(Ⅱ)──脊柱及附肢骨的比较[J]. 西南师范大学学报(自然科学版), 1995,20(02): 167-175.
[47]张耀光,王德寿. 嘉陵江鲿科鱼类骨学研究(Ⅰ)──韦伯氏器的比较[J]. 西南师范大学学报(自然科学版), 1995,20 (01): 53-58.
[48]王德寿,田怀军,蒲德永. 粗唇鮠生物学的初步研究[J]. 西南师范大学学报(自然科学版), 1995, 20(01): 59-65.
[49]金灿彪,何学福,王德寿. 大鳍鳠个体生殖力的研究[J]. 西南师范大学学报(自然科学版), 1994, 19(03): 311-315.
[50]龙祥平,王德寿,田怀军. 南方鲶、大鳍鳠鳔发育的组织学研究[J]. 水产学报, 1993, 17(04): 340-343.
[51]龙祥平,王德寿. 南方鲶仔稚鱼期齿发育的组织学和组织化学[J]. 西南师范大学学报(自然科学版), 1993,18 (03): 346-350.
[52]王德寿, 罗泉笙. 嘉陵江大鳍鳠的年龄和生长的研究[J]. 水生生物学报, 1993,17 (02): 157-165+199-200.
[53]蒋国福,王德寿. 家鸡有三个睾丸的解剖记录[J]. 四川动物, 1993, 12(01): 42.
[54]王德寿,杨松林. 大鳍鳠的可量性状、长重关系和肥满度[J]. 西南师范大学学报(自然科学版), 1992,17 (04): 510-515.
[55]王德寿,罗泉笙. 大鳍鳠的繁殖生物学研究[J]. 水产学报, 1992, 16(01): 50-59.
[56]张耀光,王德寿,罗泉笙. 大鳍鳠的胚胎发育[J]. 西南师范大学学报(自然科学版), 1991, 16(02): 223-229.
[57]王德寿,罗泉笙,刘少英. 大鳍鳠头骨形态的观察[J]. 西南师范大学学报(自然科学版), 1990,15 (03): 399-404.
[58]王德寿. 南方大口鲶颅骨形态的研究[J]. 西南师范大学学报(自然科学版), 1987, (01): 130-137.
会议论文:
[1]李明辉, 孙运侣, 赵九娥, 杨惠惠, 江东能 & 王德寿. (2015). TGF-β家族成员在罗非鱼性别决定中的作用研究. (eds.) 遗传多样性:前沿与挑战——中国的遗传学研究(2013-2015)——2015中国遗传学会大会论文摘要汇编 (pp.183).
[2]王德寿*. (2015). 基因组时代养殖鱼类性别可塑性与性控育种研究. (eds.) 遗传多样性:前沿与挑战——中国的遗传学研究(2013-2015)——2015中国遗传学会大会论文摘要汇编 (pp.189).
[3]陶文静, 江东能, 刘治隆 & 王德寿. (2015). 罗非鱼近缘种不同性别决定方式的分子机制研究. (eds.) 遗传多样性:前沿与挑战——中国的遗传学研究(2013-2015)——2015中国遗传学会大会论文摘要汇编 (pp.211).
[4]谢庆平, 何雪, 隋伊宁 & 王德寿. (2015). 利用CRISPR/Cas9敲除尼罗罗非鱼sf-1基因导致性腺发育缺陷及双向性逆转. (eds.) 遗传多样性:前沿与挑战——中国的遗传学研究(2013-2015)——2015中国遗传学会大会论文摘要汇编 (pp.224).
[5]孙丽娜, 陶文静, 石红娟, 陈立黎 & 王德寿. (2015). 罗非鱼次发性逆转的机制研究. (eds.) 遗传多样性:前沿与挑战——中国的遗传学研究(2013-2015)——2015中国遗传学会大会论文摘要汇编 (pp.255).
[6]于祥国, 吴利敏, 刘刚, 王德寿 & 周林燕. (2012). R-spondins基因在硬骨鱼类卵巢分化中的作用研究. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编 (pp.175-176).
[7]孙运侣, 曾圣, 叶凯, 杨超 & 王德寿. (2012). FGF9/16/20亚家族在尼罗罗非鱼雌性生殖细胞发育中的作用. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编 (pp.173-174).
[8]吴利敏, 周林燕 & 王德寿. (2012). Rspo1/β-catenin信号通路在尼罗罗非鱼卵巢分化中的作用. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编 (pp.174-175).
[9]周林燕, 吴利敏, 于祥国, 刘刚 & 王德寿. (2012). R-spondin1(Rspo1)/β-catenin信号通路在硬骨鱼类雌性性别决定和分化中的作用研究. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编 (pp.175).
[10]陶文静,袁静,周林燕,孙丽娜,杨世杰,孙运侣... & 王德寿. (2012). 对8个尼罗罗非鱼性腺的转录组研究揭示雌性默认通路及雌激素对于性别决定与维持过程的重要作用. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编 (pp.176-177).
[11]汪海, 李明辉, 王婷茹 & 王德寿. (2012). 尼罗罗非鱼Dmrt家族分析及Dmrt6的克隆、表达与功能初步研究. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编 (pp.178-179).
[12]李明辉, 吴风瑞, 顾源, 王婷茹 & 王德寿. (2012). 尼罗罗非鱼性腺中Igf3调节类固醇酶相关基因及关键转录因子的表达. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2012年学术研讨会论文摘要汇编 (pp.179-180).
[13]王德寿. (2011). 转基因与精原细胞移植技术相结合快速繁育名优养殖鱼类. (eds.) 新观点新学说学术沙龙文集59:转基因水产动植物的发展机遇与挑战 (pp.42-45+125-126).
[14]黄宝锋, 刘智皓, 吴风瑞 & 王德寿. (2008). 性类固醇激素对南方鲇性别分化的影响. (eds.) 中国鱼类学会2008学术研讨会论文摘要汇编 (pp.86).
[15]刘智皓, 吴风瑞 & 王德寿. (2006). 南方鲇Dmrtl及其亚型cDNA的克隆和表达的研究. (eds.) 中国海洋湖沼动物学会鱼类学分会第七届会员代表大会暨朱元鼎教授诞辰110周年庆学术研讨会学术论文摘要集 (pp.53).
[16]张修月 & 王德寿. (2004). 南方鲶受精细胞学观察. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2004年学术研讨会摘要汇编 (pp.23).
[17]黄希贵, 章力 & 王德寿. (2004). 南方鲶两种生长激素受体的克隆和系统发生分析——鱼类存在两种由不同基因编码的生长激素受体. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2004年学术研讨会摘要汇编 (pp.56-57).
[18]周林燕, 长滨嘉孝 & 王德寿. (2004). 罗非鱼(Oreochromis niloticus)三种17β-羟类固醇脱氢酶(17β-HSD1,17β-HSD8,17β-HSD12)的克隆、表达及酶活性鉴定. (eds.) 中国海洋湖沼学会鱼类学分会、中国动物学会鱼类学分会2004年学术研讨会摘要汇编 (pp.72-73).
荣誉奖励:
1、重庆市有突出贡献的中青年专家。
2、国务院政府特殊津贴获得者。
3、2010年,获重庆市自然科学一等奖 1项(排名第二)。
4、获重庆市自然科学三等奖 1项(排名第一)。
5、1998年,获重庆市科技进步一等奖 1项(排名第六)。
6、获得重庆市高等教育教学成果二等奖 1项(排名第五)。
7、领衔申报获准动物学重庆市市级精品课程。
8、鱼类生殖和发育教育部创新团队方向带头人。
9、重庆市第一、第二届水产学学术技术带头人。
10、2005年,重庆市高等教育教学成果二等奖。
研究团队负责人及团队简介:
团队负责人王德寿教授,西南大学生命科学学院博士生导师,国务院政府特殊津贴获得者,中国鱼类学会副理事长、中国动物学会比较内分泌学分会理事、重庆市有突出贡献中青年专家、重庆市水产学学术技术带头人。淡水鱼类资源与生殖发育教育部重点实验室主任、重庆市高校高水平创新平台主任。获重庆市自然科学一、三等奖和科技进步一等奖等奖项。
团队主要以罗非鱼、鲈鱼和南方鲇等为对象从事鱼类优良性状的遗传解析,在鱼类性别决定与分化的分子机制和罗非鱼性控育种研究处于世界领先水平。通过基因编辑大规模开展罗非鱼新种质创制,实现对性别、体色、生长等性状的控制。率先在罗非鱼建立基因编辑平台,建立100余个基因突变系,使罗非鱼成为唯一规模化开展基因编辑的养殖鱼类。
创制背景:
产业需求:罗非鱼是世界性养殖鱼类,中国是罗非鱼第一大生产国和出口国。
科研问题:种质退化、全雄苗种依赖激素诱导,成为我国罗非鱼产业发展“卡脖子”问题。
创制规划:
创制目标:到2027年创制素材500份,获发明专利10项;申报新品种1-2个。
成果目标:获得省部级二等及以上科研奖励1-2项。
产业合作:与2-3家水产养殖企业协同攻关,建立产学研和育繁推联合体,推动新型遗传素材育种进程和推广应用。
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